7A17

Crystal structure of the 5-phosphatase domain of Synaptojanin1 bound to its substrate diC8-PI(3,4,5)P3 in complex with a nanobody

Summary for 7A17

Entry DOI	10.2210/pdb7a17/pdb
Descriptor	Isoform 2 of Synaptojanin-1, Nanobody 13015, (2R)-3-{[(R)-{[(1S,2S,3R,4S,5S,6S)-2,6-dihydroxy-3,4,5-tris(phosphonooxy)cyclohexyl]oxy}(hydroxy)phosphoryl]oxy}propane -1,2-diyl dioctanoate, ... (10 entities in total)
Functional Keywords	inositol polyphosphate 5-phosphatase, phosphoinositide, enzyme-substrate complex, catalytic mechanism, parkinson's disease, epilepsy, hydrolase
Biological source	Homo sapiens (Human) More
Total number of polymer chains	6
Total formula weight	160279.34
Authors	Paesmans, J.,Galicia, C.,Martin, E.,Versees, W. (deposition date: 2020-08-12, release date: 2020-12-30, Last modification date: 2024-01-31)
Primary citation	Paesmans, J.,Martin, E.,Deckers, B.,Berghmans, M.,Sethi, R.,Loeys, Y.,Pardon, E.,Steyaert, J.,Verstreken, P.,Galicia, C.,Versees, W. A structure of substrate-bound Synaptojanin1 provides new insights in its mechanism and the effect of disease mutations. Elife, 9:-, 2020 Cited by PubMed: 33349335 DOI: 10.7554/eLife.64922 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.73 Å)

Structure validation