6U6F
The crystal structure of anti-apoptotic Mcl-1 protein in complex with 2, 5-substituted benzoic acid inhibitor 21
Summary for 6U6F
| Entry DOI | 10.2210/pdb6u6f/pdb |
| Related | 6U63 6U64 6U65 6U67 |
| Descriptor | Induced myeloid leukemia cell differentiation protein Mcl-1, 2-[({4-[(4-tert-butylphenyl)methyl]piperazin-1-yl}sulfonyl)amino]-5-[(2-phenylethyl)sulfanyl]benzoic acid (3 entities in total) |
| Functional Keywords | apoptosis, mcl1, bfl1/a1, protein-protein interaction, bh3 mimetics, cancer, apoptosis-inhibitor complex, apoptosis/inhibitor |
| Biological source | Homo sapiens (Human) |
| Total number of polymer chains | 3 |
| Total formula weight | 55785.74 |
| Authors | Yang, Y.,Stuckey, J.A.,Nikolovska-Coleska, Z. (deposition date: 2019-08-29, release date: 2020-03-04, Last modification date: 2023-10-11) |
| Primary citation | Kump, K.J.,Miao, L.,Mady, A.S.A.,Ansari, N.H.,Shrestha, U.K.,Yang, Y.,Pal, M.,Liao, C.,Perdih, A.,Abulwerdi, F.A.,Chinnaswamy, K.,Meagher, J.L.,Carlson, J.M.,Khanna, M.,Stuckey, J.A.,Nikolovska-Coleska, Z. Discovery and Characterization of 2,5-Substituted Benzoic Acid Dual Inhibitors of the Anti-apoptotic Mcl-1 and Bfl-1 Proteins. J.Med.Chem., 63:2489-2510, 2020 Cited by PubMed Abstract: Anti-apoptotic Bcl-2 family proteins are overexpressed in a wide spectrum of cancers and have become well validated therapeutic targets. Cancer cells display survival dependence on individual or subsets of anti-apoptotic proteins that could be effectively targeted by multimodal inhibitors. We designed a 2,5-substituted benzoic acid scaffold that displayed equipotent binding to Mcl-1 and Bfl-1. Structure-based design was guided by several solved cocrystal structures with Mcl-1, leading to the development of compound , which binds both Mcl-1 and Bfl-1 with values of 100 nM and shows appreciable selectivity over Bcl-2/Bcl-xL. The selective binding profile of was translated to on-target cellular activity in model lymphoma cell lines. These studies lay a foundation for developing more advanced dual Mcl-1/Bfl-1 inhibitors that have potential to provide greater single agent efficacy and broader coverage to combat resistance in several types of cancer than selective Mcl-1 inhibitors alone. PubMed: 31971799DOI: 10.1021/acs.jmedchem.9b01442 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.9 Å) |
Structure validation
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