6MII

Crystal structure of minichromosome maintenance protein MCM/DNA complex

Summary for 6MII

• Entry DOI: 10.2210/pdb6mii/pdb
• Descriptor: Minichromosome maintenance protein MCM, DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3'), [[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-tris(fluoranyl)beryllium, ... (7 entities in total)
• Functional Keywords: mcm, dna, helicase, aaa+, hydrolase-dna complex, hydrolase/dna
• Biological source: Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2); More
• Total number of polymer chains: 7
• Total formula weight: 418753.61

Authors

Enemark, E.J.,Meagher, M.,Epling, L.B. (deposition date: 2018-09-19, release date: 2019-07-24, Last modification date: 2023-10-11)

Primary citation

Meagher, M.,Epling, L.B.,Enemark, E.J.
DNA translocation mechanism of the MCM complex and implications for replication initiation.
Nat Commun, 10:3117-3117, 2019

PubMed Abstract: The DNA translocation activity of the minichromosome maintenance (MCM) complex powers DNA strand separation of the replication forks of eukaryotes and archaea. Here we illustrate an atomic level mechanism for this activity with a crystal structure of an archaeal MCM hexamer bound to single-stranded DNA and nucleotide cofactors. Sequence conservation indicates this rotary mechanism is fully possible for all eukaryotes and archaea. The structure definitively demonstrates the ring orients during translocation with the N-terminal domain leading, indicating that the translocation activity could also provide the physical basis of replication initiation where a double-hexamer idly encircling double-stranded DNA transforms to single-hexamers that encircle only one strand. In this mechanism, each strand binds to the N-terminal tier of one hexamer and the AAA+ tier of the other hexamer such that one ring pulls on the other, aligning equivalent interfaces to enable each hexamer to pull its translocation strand outside of the opposing hexamer.

PubMed: 31308367
DOI: 10.1038/s41467-019-11074-3

Experimental method

X-RAY DIFFRACTION (3.15 Å)