6MII
Crystal structure of minichromosome maintenance protein MCM/DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-26 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 186.665, 186.665, 281.489 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.698 - 3.150 |
R-factor | 0.1908 |
Rwork | 0.188 |
R-free | 0.24130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 4R7Y & 2VL6 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.044 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.260 |
High resolution limit [Å] | 3.150 | 3.150 |
Rmerge | 0.119 | 1.789 |
Rmeas | 0.036 | 2.007 |
Rpim | 0.125 | 0.877 |
Number of reflections | 84725 | 7600 |
<I/σ(I)> | 17.6 | 0.5 |
Completeness [%] | 98.0 | 89.6 |
Redundancy | 11.1 | 4.5 |
CC(1/2) | 0.245 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 50 mM MES, pH 6.0, 100 mM sodium chloride, 20 mM magnesium chloride, 9% PEG8000 |