6AI6

Crystal structure of SpCas9-NG

Summary for 6AI6

Entry DOI	10.2210/pdb6ai6/pdb
Descriptor	CRISPR-associated endonuclease Cas9/Csn1, RNA (81-MER), DNA (28-MER), ... (8 entities in total)
Functional Keywords	nuclease, hydrolase-rna-dna complex, hydrolase, hydrolase/rna/dna
Biological source	Streptococcus pyogenes serotype M1 More
Total number of polymer chains	4
Total formula weight	197022.33
Authors	Nishimasu, H.,Hirano, S.,Ishitani, R.,Nureki, O. (deposition date: 2018-08-21, release date: 2018-10-31, Last modification date: 2023-11-22)
Primary citation	Nishimasu, H.,Shi, X.,Ishiguro, S.,Gao, L.,Hirano, S.,Okazaki, S.,Noda, T.,Abudayyeh, O.O.,Gootenberg, J.S.,Mori, H.,Oura, S.,Holmes, B.,Tanaka, M.,Seki, M.,Hirano, H.,Aburatani, H.,Ishitani, R.,Ikawa, M.,Yachie, N.,Zhang, F.,Nureki, O. Engineered CRISPR-Cas9 nuclease with expanded targeting space Science, 361:1259-1262, 2018 Cited by PubMed: 30166441 DOI: 10.1126/science.aas9129 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.7 Å)

Structure validation