6AI6
Crystal structure of SpCas9-NG
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1372 | 159226.5 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) | Streptococcus pyogenes serotype M1 | SpCas9,SpyCas9 |
| 2 | B (B) | RNA (81-MER) | polymer | 81 | 26209.6 | 1 | Streptococcus pyogenes | ||
| 3 | C (C) | DNA (28-MER) | polymer | 28 | 8463.5 | 1 | Streptococcus pyogenes | ||
| 4 | D (D) | DNA (8-MER) | polymer | 8 | 2472.6 | 1 | Streptococcus pyogenes | ||
| 5 | E, F, G, H, I... (A, B) | POTASSIUM ION | non-polymer | 39.1 | 10 | Chemie (K) | |||
| 6 | M, S, T (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 7 | N, O, P (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 3 | Chemie (EDO) | |||
| 8 | U, V, W (A, B, C) | water | water | 18.0 | 36 | Chemie (HOH) |
Sequence modifications
A: 1 - 1368 (UniProt: Q99ZW2)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Gly -1 | - | expression tag |
| His 0 | - | expression tag |
| Ala 863 | Asn 863 | engineered mutation |
| Arg 1111 | Leu 1111 | engineered mutation |
| Val 1135 | Asp 1135 | engineered mutation |
| Arg 1218 | Gly 1218 | engineered mutation |
| Phe 1219 | Glu 1219 | engineered mutation |
| Arg 1322 | Ala 1322 | engineered mutation |
| Val 1335 | Arg 1335 | engineered mutation |
| Arg 1337 | Thr 1337 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 196372.2 | |
| Non-Polymers* | Number of molecules | 16 |
| Total formula weight | 650.1 | |
| All* | Total formula weight | 197022.3 |
