6AI6

Crystal structure of SpCas9-NG

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003676	molecular_function	nucleic acid binding
A	0003677	molecular_function	DNA binding
A	0003723	molecular_function	RNA binding
A	0004518	molecular_function	nuclease activity
A	0004519	molecular_function	endonuclease activity
A	0004520	molecular_function	DNA endonuclease activity
A	0004527	molecular_function	exonuclease activity
A	0008408	molecular_function	3'-5' exonuclease activity
A	0016787	molecular_function	hydrolase activity
A	0043571	biological_process	maintenance of CRISPR repeat elements
A	0046872	molecular_function	metal ion binding
A	0051607	biological_process	defense response to virus

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue K A 1401

Chain	Residue
A	LEU623
A	THR624
A	PHE626
A	TYR656

---

site_id	AC2
Number of Residues	4
Details	binding site for residue K A 1402

Chain	Residue
A	GLY361
A	TYR362
A	GLY365
A	ALA367

---

site_id	AC3
Number of Residues	1
Details	binding site for residue K A 1403

Chain	Residue
A	GLU57

---

site_id	AC4
Number of Residues	1
Details	binding site for residue K A 1404

Chain	Residue
A	LYS111

---

site_id	AC5
Number of Residues	3
Details	binding site for residue K A 1405

Chain	Residue
A	ILE226
A	LEU229
A	GLU232

---

site_id	AC6
Number of Residues	3
Details	binding site for residue K A 1406

Chain	Residue
A	GLU584
A	ARG586
A	ASN588

---

site_id	AC7
Number of Residues	3
Details	binding site for residue K A 1407

Chain	Residue
A	GLN1350
A	HOH1506
B	A68

---

site_id	AC8
Number of Residues	3
Details	binding site for residue K A 1408

Chain	Residue
A	ASP364
A	GLY365
B	U22

---

site_id	AC9
Number of Residues	3
Details	binding site for residue MG A 1409

Chain	Residue
A	HOH1515
B	HOH208
B	HOH220

---

site_id	AD1
Number of Residues	3
Details	binding site for residue EDO A 1410

Chain	Residue
A	ARG461
B	U63
B	U64

---

site_id	AD2
Number of Residues	5
Details	binding site for residue EDO A 1411

Chain	Residue
A	PHE1105
A	ILE1168
A	MET1169
A	SER1172
B	A52

---

site_id	AD3
Number of Residues	7
Details	binding site for residue EDO A 1412

Chain	Residue
A	TYR136
A	ARG139
A	HIS160
A	ASN407
A	HIS415
B	G19
B	C20

---

site_id	AD4
Number of Residues	3
Details	binding site for residue K A 1413

Chain	Residue
A	THR1102
B	A65
B	U66

---

site_id	AD5
Number of Residues	1
Details	binding site for residue K B 101

Chain	Residue
B	G21

---

site_id	AD6
Number of Residues	3
Details	binding site for residue MG B 102

Chain	Residue
B	U50
B	HOH204
B	HOH219

---

site_id	AD7
Number of Residues	2
Details	binding site for residue MG B 103

Chain	Residue
B	G21
B	U22

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	17
Details	Region: {"description":"ARM","evidences":[{"source":"PubMed","id":"24505130","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	47
Details	Region: {"description":"RuvC-II","evidences":[{"source":"PubMed","id":"24505130","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Motif: {"description":"PAM substrate-binding","evidences":[{"source":"PubMed","id":"25079318","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	Active site: {"description":"For RuvC-like nuclease domain","evidences":[{"source":"PubMed","id":"24529477","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	Active site: {"description":"Proton acceptor for HNH nuclease domain","evidences":[{"source":"PubMed","id":"24529477","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	5
Details	Binding site: {"evidences":[{"source":"PubMed","id":"24505130","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---