5Y36

Cryo-EM structure of SpCas9-sgRNA-DNA ternary complex

Summary for 5Y36

• Entry DOI: 10.2210/pdb5y36/pdb
• EMDB information: 8236
• Descriptor: CRISPR-associated endonuclease Cas9/Csn1, single-guide RNA, complementary DNA strand, ... (5 entities in total)
• Functional Keywords: genome editting, cripsr-cas9, dna cleavage mechanism, hydrolase-dna-rna complex, hydrolase-rna-dna complex, hydrolase/rna/dna
• Biological source: Streptococcus pyogenes serotype M1; More
• Total number of polymer chains: 4
• Total formula weight: 215795.84

Authors

Huang, Q.,Li, G.,Huai, C. (deposition date: 2017-07-27, release date: 2017-12-06, Last modification date: 2024-03-27)

• Primary citation: Huai, C.,Li, G.,Yao, R.,Zhang, Y.,Cao, M.,Kong, L.,Jia, C.,Yuan, H.,Chen, H.,Lu, D.,Huang, Q.; Structural insights into DNA cleavage activation of CRISPR-Cas9 system; Nat Commun, 8:1375-1375, 2017; PubMed: 29123204; DOI: 10.1038/s41467-017-01496-2

Experimental method

ELECTRON MICROSCOPY (5.2 Å)