5Y36
Cryo-EM structure of SpCas9-sgRNA-DNA ternary complex
Summary for 5Y36
Entry DOI | 10.2210/pdb5y36/pdb |
EMDB information | 8236 |
Descriptor | CRISPR-associated endonuclease Cas9/Csn1, single-guide RNA, complementary DNA strand, ... (5 entities in total) |
Functional Keywords | genome editting, cripsr-cas9, dna cleavage mechanism, hydrolase-dna-rna complex, hydrolase-rna-dna complex, hydrolase/rna/dna |
Biological source | Streptococcus pyogenes serotype M1 More |
Total number of polymer chains | 4 |
Total formula weight | 215795.84 |
Authors | |
Primary citation | Huai, C.,Li, G.,Yao, R.,Zhang, Y.,Cao, M.,Kong, L.,Jia, C.,Yuan, H.,Chen, H.,Lu, D.,Huang, Q. Structural insights into DNA cleavage activation of CRISPR-Cas9 system Nat Commun, 8:1375-1375, 2017 Cited by PubMed: 29123204DOI: 10.1038/s41467-017-01496-2 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (5.2 Å) |
Structure validation
Download full validation report