5Y36
Cryo-EM structure of SpCas9-sgRNA-DNA ternary complex
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 5.2 |
| Number of Particle | 66845 |
| Software | RELION,CTFFIND3,NAMD,Rosetta |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING ONLY |
