5Y36
Cryo-EM structure of SpCas9-sgRNA-DNA ternary complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1368 | 158588.8 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) In PDB | Streptococcus pyogenes serotype M1 | SpCas9,SpyCas9 |
| 2 | B | single-guide RNA | polymer | 99 | 31890.9 | 1 | Mus musculus | ||
| 3 | C | complementary DNA strand | polymer | 41 | 12697.2 | 1 | Mus musculus | ||
| 4 | D | non-complementary DNA strand | polymer | 41 | 12546.1 | 1 | Mus musculus | ||
| 5 | A, D | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) |
Sequence modifications
A: 1 - 1368 (UniProt: Q99ZW2)
| PDB | External Database | Details |
|---|---|---|
| Ala 10 | Asp 10 | engineered mutation |
| Ala 840 | His 840 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 215722.9 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 72.9 | |
| All* | Total formula weight | 215795.8 |
