5Y36

Cryo-EM structure of SpCas9-sgRNA-DNA ternary complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003723	molecular_function	RNA binding
A	0004519	molecular_function	endonuclease activity
A	0004520	molecular_function	DNA endonuclease activity
A	0004527	molecular_function	exonuclease activity
A	0008408	molecular_function	3'-5' exonuclease activity
A	0043571	biological_process	maintenance of CRISPR repeat elements
A	0046872	molecular_function	metal ion binding
A	0051607	biological_process	defense response to virus

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue MG A 1401

Chain	Residue
A	HIS982
A	HIS983
A	ASP986
D	DA34

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site_id	AC2
Number of Residues	2
Details	binding site for residue MG A 1402

Chain	Residue
A	ASP839
A	ASP861

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site_id	AC3
Number of Residues	2
Details	binding site for residue MG D 101

Chain	Residue
A	ARG925
D	DA34

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: For RuvC-like nuclease domain => ECO:0000305|PubMed:24529477

Chain	Residue	Details
A	ALA10

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site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Proton acceptor for HNH nuclease domain => ECO:0000305|PubMed:24529477

Chain	Residue	Details
A	ALA840

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site_id	SWS_FT_FI3
Number of Residues	6
Details	BINDING: BINDING => ECO:0000269|PubMed:24505130

Chain	Residue	Details
A	ALA10
A	GLU762
A	GLU766
A	HIS983
A	HIS1297
A	ASP1328

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