5VMZ

Kaiso (ZBTB33) E535Q mutant zinc finger DNA binding domain in complex with a double CpG-methylated DNA resembling the specific Kaiso binding sequence (KBS)

Summary for 5VMZ

• Entry DOI: 10.2210/pdb5vmz/pdb
• Related: 5VMU 5VMV 5VMW 5VMX 5VMY
• Descriptor: Transcriptional regulator Kaiso, DNA (5'-D(*TP*GP*CP*TP*TP*CP*CP*(5CM)P*GP*(5CM)P*GP*AP*AP*TP*AP*AP*CP*G)-3'), DNA (5'-D(*CP*GP*TP*TP*AP*TP*TP*(5CM)P*GP*(5CM)P*GP*GP*GP*AP*AP*GP*CP*A)-3'), ... (7 entities in total)
• Functional Keywords: dna methylation, zinc finger, transcriptional regulator, transcription-dna complex, transcription/dna
• Biological source: Homo sapiens (Human); More
• Cellular location: Nucleus : Q86T24
• Total number of polymer chains: 3
• Total formula weight: 27687.82

Authors

Nikolova, E.N.,Stanfield, R.L.,Martinez-Yamout, M.A.,Dyson, H.J.,Wright, P.E. (deposition date: 2017-04-28, release date: 2018-04-04, Last modification date: 2023-10-04)

• Primary citation: Nikolova, E.N.,Stanfield, R.L.,Dyson, H.J.,Wright, P.E.; CH···O Hydrogen Bonds Mediate Highly Specific Recognition of Methylated CpG Sites by the Zinc Finger Protein Kaiso.; Biochemistry, 57:2109-2120, 2018; PubMed: 29546986; DOI: 10.1021/acs.biochem.8b00065

Experimental method

X-RAY DIFFRACTION (2.319 Å)