5VMZ
Kaiso (ZBTB33) E535Q mutant zinc finger DNA binding domain in complex with a double CpG-methylated DNA resembling the specific Kaiso binding sequence (KBS)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Transcriptional regulator Kaiso | polymer | 134 | 16216.8 | 1 | UniProt (Q86T24) | Homo sapiens (Human) | Zinc finger and BTB domain-containing protein 33 |
| 2 | B (D) | DNA (5'-D(*TP*GP*CP*TP*TP*CP*CP*(5CM)P*GP*(5CM)P*GP*AP*AP*TP*AP*AP*CP*G)-3') | polymer | 18 | 5504.6 | 1 | synthetic construct | ||
| 3 | C (E) | DNA (5'-D(*CP*GP*TP*TP*AP*TP*TP*(5CM)P*GP*(5CM)P*GP*GP*GP*AP*AP*GP*CP*A)-3') | polymer | 18 | 5584.7 | 1 | synthetic construct | ||
| 4 | D, E, F (A) | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) | |||
| 5 | G (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 6 | H (A) | L(+)-TARTARIC ACID | non-polymer | 150.1 | 1 | Chemie (TLA) | |||
| 7 | I, J, K (A, D, E) | water | water | 18.0 | 84 | Chemie (HOH) |
Sequence modifications
A: 471 - 604 (UniProt: Q86T24)
| PDB | External Database | Details |
|---|---|---|
| Gln 535 | Glu 535 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 27306.1 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 381.8 | |
| All* | Total formula weight | 27687.8 |
