5VMZ

Kaiso (ZBTB33) E535Q mutant zinc finger DNA binding domain in complex with a double CpG-methylated DNA resembling the specific Kaiso binding sequence (KBS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	C 2 2 2
Unit cell lengths	44.409, 183.487, 104.003
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.240 - 2.319
R-factor	0.1704
Rwork	0.167
R-free	0.19850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f6n
RMSD bond length	0.009
RMSD bond angle	1.161
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.240	2.400
High resolution limit [Å]	2.319	2.320
Rmerge	0.063	0.309
Number of reflections	18669	1629
<I/σ(I)>	18.9	4.53
Completeness [%]	98.4	87.77
Redundancy	6.5	5.5
CC(1/2)	0.998	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	295	0.1 M Disodium tartrate, 20 % PEG 3350