5VLJ

Cryo-EM structure of yeast cytoplasmic dynein with Walker B mutation at AAA3 in presence of ATP-VO4

Summary for 5VLJ

• Entry DOI: 10.2210/pdb5vlj/pdb
• Related: 5VH9
• EMDB information: 8673 8706
• Descriptor: Dynein heavy chain, cytoplasmic, Nuclear distribution protein PAC1 (2 entities in total)
• Functional Keywords: cytoplasmic dynein, lis1, motor protein
• Biological source: Saccharomyces cerevisiae (Baker's yeast); More
• Total number of polymer chains: 3
• Total formula weight: 353957.15

Authors

Cianfrocco, M.A.,DeSantis, M.E.,Htet, Z.M.,Tran, P.T.,Reck-Peterson, S.L.,Leschziner, A.E. (deposition date: 2017-04-25, release date: 2017-09-06, Last modification date: 2024-10-30)

Primary citation

DeSantis, M.E.,Cianfrocco, M.A.,Htet, Z.M.,Tran, P.T.,Reck-Peterson, S.L.,Leschziner, A.E.
Lis1 Has Two Opposing Modes of Regulating Cytoplasmic Dynein.
Cell, 170:1197-1208.e12, 2017

PubMed Abstract: Regulation is central to the functional versatility of cytoplasmic dynein, a motor involved in intracellular transport, cell division, and neurodevelopment. Previous work established that Lis1, a conserved regulator of dynein, binds to its motor domain and induces a tight microtubule-binding state in dynein. The work we present here-a combination of biochemistry, single-molecule assays, and cryoelectron microscopy-led to the surprising discovery that Lis1 has two opposing modes of regulating dynein, being capable of inducing both low and high affinity for the microtubule. We show that these opposing modes depend on the stoichiometry of Lis1 binding to dynein and that this stoichiometry is regulated by the nucleotide state of dynein's AAA3 domain. The low-affinity state requires Lis1 to also bind to dynein at a novel conserved site, mutation of which disrupts Lis1's function in vivo. We propose a new model for the regulation of dynein by Lis1.

PubMed: 28886386
DOI: 10.1016/j.cell.2017.08.037

Experimental method

ELECTRON MICROSCOPY (10.5 Å)