5UPK

CDC42 binds PAK4 via an extended GTPase-effector interface - 3 peptide: PAK4cat, PAK4-N45, CDC42

Summary for 5UPK

• Entry DOI: 10.2210/pdb5upk/pdb
• Related: 5UPL
• Descriptor: Serine/threonine-protein kinase PAK 4, Cell division control protein 42 homolog, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, ... (7 entities in total)
• Functional Keywords: transferase, gtpase, kinase, crib
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 3
• Total formula weight: 66558.94

Authors

Ha, B.H.,Boggon, T.J. (deposition date: 2017-02-03, release date: 2017-12-27, Last modification date: 2024-10-16)

Primary citation

Ha, B.H.,Boggon, T.J.
CDC42 binds PAK4 via an extended GTPase-effector interface.
Proc. Natl. Acad. Sci. U.S.A., 115:531-536, 2018

PubMed Abstract: The p21-activated kinase (PAK) group of serine/threonine kinases are downstream effectors of RHO GTPases and play important roles in regulation of the actin cytoskeleton, cell growth, survival, polarity, and development. Here we probe the interaction of the type II PAK, PAK4, with RHO GTPases. Using solution scattering we find that the full-length PAK4 heterodimer with CDC42 adopts primarily a compact organization. X-ray crystallography reveals the molecular nature of the interaction between PAK4 and CDC42 and shows that in addition to the canonical PAK4 CDC42/RAC interactive binding (CRIB) domain binding to CDC42 there are unexpected contacts involving the PAK4 kinase C-lobe, CDC42, and the PAK4 polybasic region. These additional interactions modulate kinase activity and increase the binding affinity of CDC42 for full-length PAK4 compared with the CRIB domain alone. We therefore show that the interaction of CDC42 with PAK4 can influence kinase activity in a previously unappreciated manner.

PubMed: 29295922
DOI: 10.1073/pnas.1717437115

Experimental method

X-RAY DIFFRACTION (2.4 Å)