5UPK
CDC42 binds PAK4 via an extended GTPase-effector interface - 3 peptide: PAK4cat, PAK4-N45, CDC42
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97920 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.465, 61.621, 85.791 |
Unit cell angles | 90.00, 91.57, 90.00 |
Refinement procedure
Resolution | 50.010 - 2.400 |
R-factor | 0.22023 |
Rwork | 0.218 |
R-free | 0.26364 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fij |
RMSD bond length | 0.008 |
RMSD bond angle | 1.351 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.163 | |
Rpim | 0.912 | |
Number of reflections | 24144 | |
<I/σ(I)> | 12 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.9 | 10.6 |
CC(1/2) | 0.311 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1M HEPES pH 7.5, 22% PEG1,000 |