5T8D

Engineered variant of I-OnuI meganuclease targeting the HIV integrase gene; harbors 47 point mutations relative to wild-type I-OnuI

Summary for 5T8D

• Entry DOI: 10.2210/pdb5t8d/pdb
• Related: 5V0Q
• Descriptor: I-OnuI_e-vHIVInt_v2, DNA (26-MER), CALCIUM ION, ... (5 entities in total)
• Functional Keywords: meganuclease, engineered protein, dna complex, homing endonuclease, hydrolase-dna complex, hydrolase/dna
• Biological source: synthetic construct; More
• Total number of polymer chains: 3
• Total formula weight: 50521.32

Authors

Stoddard, B.L.,Werther, R.,Lambert, A.R. (deposition date: 2016-09-07, release date: 2017-05-03, Last modification date: 2023-10-04)

• Primary citation: Niyonzima, N.,Lambert, A.R.,Werther, R.,De Silva Feelixge, H.,Roychoudhury, P.,Greninger, A.L.,Stone, D.,Stoddard, B.L.,Jerome, K.R.; Tuning DNA binding affinity and cleavage specificity of an engineered gene-targeting nuclease via surface display, flow cytometry and cellular analyses.; Protein Eng.Des.Sel., 30:503-522, 2017; PubMed: 28873986; DOI: 10.1093/protein/gzx037

Experimental method

X-RAY DIFFRACTION (2.15 Å)