5NMV

Crystal structure of 2F22 Fab fragment against TFPI1

Summary for 5NMV

Entry DOI	10.2210/pdb5nmv/pdb
Descriptor	Fab light chain, Fab heavy chain, Tissue factor pathway inhibitor, ... (5 entities in total)
Functional Keywords	fab, immune system
Biological source	Mus More
Cellular location	Isoform Alpha: Secreted. Isoform Beta: Microsome membrane ; Lipid-anchor, GPI-anchor : P10646
Total number of polymer chains	3
Total formula weight	57223.83
Authors	Svensson, L.A.,Petersen, H.H. (deposition date: 2017-04-07, release date: 2018-03-28, Last modification date: 2024-11-13)
Primary citation	Augustsson, C.,Svensson, A.,Kjaer, B.,Chao, T.Y.,Wenjuan, X.,Krogh, B.O.,Breinholt, J.,Clausen, J.T.,Hilden, I.,Petersen, H.H.,Petersen, L.C. Factor Xa and VIIa inhibition by tissue factor pathway inhibitor is prevented by a monoclonal antibody to its Kunitz-1 domain. J. Thromb. Haemost., 16:893-904, 2018 Cited by PubMed Abstract: Essentials Activated FVII (FVIIa) and FX (FXa) are inhibited by tissue factor pathway inhibitor (TFPI). A monoclonal antibody, mAb2F22, was raised against the N-terminal fragment of TFPI (1-79). mAb2F22 bound exclusively to the K1 domain of TFPI (K ∼1 nm) and not to the K2 domain. mAb2F22 interfered with inhibition of both FVIIa and FXa activities and restored clot formation. PubMed: 29532595 DOI: 10.1111/jth.14000 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.65 Å)

Structure validation