5NFV
Crystal structure of catalytically inactive FnCas12 mutant bound to an R-loop structure containing a pre-crRNA mimic and full-length DNA target
Summary for 5NFV
Entry DOI | 10.2210/pdb5nfv/pdb |
Descriptor | CRISPR-associated endonuclease Cpf1, pre-crRNA, DNA target strand, ... (7 entities in total) |
Functional Keywords | crispr, cas, cpf1, cas12a, nuclease, genome editing, r-loop, crrna, ruvc, hydrolase |
Biological source | Francisella tularensis subsp. novicida (strain U112) More |
Total number of polymer chains | 4 |
Total formula weight | 190495.05 |
Authors | |
Primary citation | Swarts, D.C.,van der Oost, J.,Jinek, M. Structural Basis for Guide RNA Processing and Seed-Dependent DNA Targeting by CRISPR-Cas12a. Mol. Cell, 66:221-233.e4, 2017 Cited by PubMed: 28431230DOI: 10.1016/j.molcel.2017.03.016 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.501 Å) |
Structure validation
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