5NFV
Crystal structure of catalytically inactive FnCas12 mutant bound to an R-loop structure containing a pre-crRNA mimic and full-length DNA target
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-08-26 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.00768 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 82.421, 143.703, 84.628 |
Unit cell angles | 90.00, 94.16, 90.00 |
Refinement procedure
Resolution | 46.568 - 2.501 |
R-factor | 0.207 |
Rwork | 0.205 |
R-free | 0.23760 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 0.592 |
Data reduction software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.568 | 2.650 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.062 | 0.878 |
Number of reflections | 131823 | |
<I/σ(I)> | 14.6 | 1.49 |
Completeness [%] | 98.5 | 96.5 |
Redundancy | 3.5 | 3.5 |
CC(1/2) | 0.999 | 0.589 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M Bis-Tris propane (pH 6.5), 0.2 M KSCN, 20% (w/v) polyethylene glycol 3,350 |