5NFV
Crystal structure of catalytically inactive FnCas12 mutant bound to an R-loop structure containing a pre-crRNA mimic and full-length DNA target
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0003677 | molecular_function | DNA binding |
A | 0003723 | molecular_function | RNA binding |
A | 0004519 | molecular_function | endonuclease activity |
A | 0004530 | molecular_function | deoxyribonuclease I activity |
A | 0006259 | biological_process | DNA metabolic process |
A | 0016829 | molecular_function | lyase activity |
A | 0033898 | molecular_function | Bacillus subtilis ribonuclease activity |
A | 0051607 | biological_process | defense response to virus |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 6 |
Details | binding site for residue MG A 1401 |
Chain | Residue |
A | ARG800 |
A | HOH1511 |
B | A-3 |
B | HOH208 |
B | HOH215 |
B | HOH216 |
site_id | AC2 |
Number of Residues | 5 |
Details | binding site for residue MG A 1402 |
Chain | Residue |
A | TYR248 |
A | ASN270 |
A | LEU66 |
A | SER67 |
A | VAL69 |
site_id | AC3 |
Number of Residues | 9 |
Details | binding site for residue B3P A 1403 |
Chain | Residue |
A | SER12 |
A | GLU758 |
A | PHE888 |
A | SER890 |
A | SER891 |
A | HOH1501 |
C | DT-1 |
C | HOH103 |
D | DC2 |
site_id | AC4 |
Number of Residues | 6 |
Details | binding site for residue MG B 101 |
Chain | Residue |
B | HOH202 |
B | HOH211 |
B | HOH212 |
B | HOH214 |
B | HOH217 |
B | HOH219 |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 3 |
Details | ACT_SITE: For pre-crRNA processing => ECO:0000305|PubMed:28431230 |
Chain | Residue | Details |
A | HIS843 | |
A | LYS852 | |
A | LYS869 |
site_id | SWS_FT_FI2 |
Number of Residues | 3 |
Details | ACT_SITE: For DNase activity of RuvC domain => ECO:0000305|PubMed:26422227 |
Chain | Residue | Details |
A | ASP917 | |
A | GLN1006 | |
A | ASP1255 |
site_id | SWS_FT_FI3 |
Number of Residues | 1 |
Details | SITE: Binds crRNA alone and in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 |
Chain | Residue | Details |
A | THR16 |
site_id | SWS_FT_FI4 |
Number of Residues | 1 |
Details | SITE: Binds target strand DNA => ECO:0000269|PubMed:28431230 |
Chain | Residue | Details |
A | LYS131 |
site_id | SWS_FT_FI5 |
Number of Residues | 1 |
Details | SITE: Binds crRNA in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230 |
Chain | Residue | Details |
A | THR295 |
site_id | SWS_FT_FI6 |
Number of Residues | 3 |
Details | SITE: Binds DNA in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230 |
Chain | Residue | Details |
A | LYS320 | |
A | SER334 | |
A | LYS589 |
site_id | SWS_FT_FI7 |
Number of Residues | 1 |
Details | SITE: Caps the crRNA-target DNA heteroduplex => ECO:0000305|PubMed:28431230 |
Chain | Residue | Details |
A | TYR410 |
site_id | SWS_FT_FI8 |
Number of Residues | 1 |
Details | SITE: Binds DNA protospacer adjacent motif (PAM) => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 |
Chain | Residue | Details |
A | LYS613 |
site_id | SWS_FT_FI9 |
Number of Residues | 1 |
Details | SITE: Binds Target strand DNA => ECO:0000269|PubMed:28431230 |
Chain | Residue | Details |
A | LYS667 |
site_id | SWS_FT_FI10 |
Number of Residues | 1 |
Details | SITE: Binds PAM => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 |
Chain | Residue | Details |
A | LYS671 |
site_id | SWS_FT_FI11 |
Number of Residues | 2 |
Details | SITE: Binds Target strand DNA => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 |
Chain | Residue | Details |
A | LYS677 | |
A | LYS823 |
site_id | SWS_FT_FI12 |
Number of Residues | 1 |
Details | SITE: Binds Target strand DNA; via amide nitrogen => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 |
Chain | Residue | Details |
A | GLY826 |
site_id | SWS_FT_FI13 |
Number of Residues | 1 |
Details | SITE: Binds crRNA => ECO:0000269|PubMed:28431230 |
Chain | Residue | Details |
A | ARG833 |
site_id | SWS_FT_FI14 |
Number of Residues | 1 |
Details | SITE: Stabilizes transition state for pre-crRNA processing => ECO:0000305|PubMed:28431230 |
Chain | Residue | Details |
A | LYS852 |
site_id | SWS_FT_FI15 |
Number of Residues | 2 |
Details | SITE: Binds DNA in crRNA-target DNA heteroduplex => ECO:0000269|PubMed:28431230, ECO:0000269|PubMed:28562584 |
Chain | Residue | Details |
A | LYS1026 | |
A | THR1063 |