5NFV
Crystal structure of catalytically inactive FnCas12 mutant bound to an R-loop structure containing a pre-crRNA mimic and full-length DNA target
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease Cpf1 | polymer | 1302 | 152195.1 | 1 | UniProt (A0Q7Q2) Pfam (PF18501) Pfam (PF21918) Pfam (PF22222) Pfam (PF18516) Pfam (PF18510) | Francisella tularensis subsp. novicida (strain U112) | FnCpf1 |
| 2 | B (B) | pre-crRNA | polymer | 46 | 14660.7 | 1 | synthetic construct | ||
| 3 | C (C) | DNA target strand | polymer | 38 | 11666.5 | 1 | synthetic construct | ||
| 4 | D (D) | DNA non-target strand | polymer | 38 | 11617.5 | 1 | synthetic construct | ||
| 5 | E, F, H (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 6 | G (A) | 2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 282.3 | 1 | Chemie (B3P) | |||
| 7 | I, J, K, L (A, B, C, D) | water | water | 18.0 | 42 | Chemie (HOH) |
Sequence modifications
A: 2 - 1300 (UniProt: A0Q7Q2)
| PDB | External Database | Details |
|---|---|---|
| Ser -1 | - | expression tag |
| Asn 0 | - | expression tag |
| Ala 1 | - | expression tag |
| Gln 1006 | Glu 1006 | engineered mutation |
| Ala 1218 | Arg 1218 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 190139.8 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 355.2 | |
| All* | Total formula weight | 190495.1 |
