5M3O

HTRA2 A141S mutant structure

5M3O の概要

• エントリーDOI: 10.2210/pdb5m3o/pdb
• 関連するPDBエントリー: 5m3n 5tny 5tnz 5to0 5to1
• 分子名称: Serine protease HTRA2, mitochondrial, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID (3 entities in total)
• 機能のキーワード: serine protease parkinson disease mitochondria pdz domain dynamics, hydrolase
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36456.38

構造登録者

Merski, M.,Barbosa Pereira, P.J.,Macedo-Ribeiro, S. (登録日: 2016-10-15, 公開日: 2017-10-25, 最終更新日: 2024-01-17)

主引用文献

Merski, M.,Moreira, C.,Abreu, R.M.,Ramos, M.J.,Fernandes, P.A.,Martins, L.M.,Pereira, P.J.B.,Macedo-Ribeiro, S.
Molecular motion regulates the activity of the Mitochondrial Serine Protease HtrA2.
Cell Death Dis, 8:e3119-e3119, 2017

PubMed Abstract: HtrA2 (high-temperature requirement 2) is a human mitochondrial protease that has a role in apoptosis and Parkinson's disease. The structure of HtrA2 with an intact catalytic triad was determined, revealing a conformational change in the active site loops, involving mainly the regulatory LD loop, which resulted in burial of the catalytic serine relative to the previously reported structure of the proteolytically inactive mutant. Mutations in the loops surrounding the active site that significantly restricted their mobility, reduced proteolytic activity both in vitro and in cells, suggesting that regulation of HtrA2 activity cannot be explained by a simple transition to an activated conformational state with enhanced active site accessibility. Manipulation of solvent viscosity highlighted an unusual bi-phasic behavior of the enzymatic activity, which together with MD calculations supports the importance of motion in the regulation of the activity of HtrA2. HtrA2 is an unusually thermostable enzyme (T=97.3 °C), a trait often associated with structural rigidity, not dynamic motion. We suggest that this thermostability functions to provide a stable scaffold for the observed loop motions, allowing them a relatively free conformational search within a rather restricted volume.

PubMed: 29022916
DOI: 10.1038/cddis.2017.487

実験手法

X-RAY DIFFRACTION (1.7 Å)