5GTM

Modified human MxA, nucleotide-free form

Summary for 5GTM

• Entry DOI: 10.2210/pdb5gtm/pdb
• Descriptor: Interferon-induced GTP-binding protein Mx1 (2 entities in total)
• Functional Keywords: hydrolysis, antiviral activity, antiviral protein
• Biological source: Homo sapiens (Human)
• Cellular location: Cytoplasm . Isoform 2: Cytoplasm : P20591
• Total number of polymer chains: 2
• Total formula weight: 136859.42

Authors

Chen, Y.,Gao, S. (deposition date: 2016-08-22, release date: 2017-05-31, Last modification date: 2023-11-08)

Primary citation

Chen, Y.,Zhang, L.,Graf, L.,Yu, B.,Liu, Y.,Kochs, G.,Zhao, Y.,Gao, S.
Conformational dynamics of dynamin-like MxA revealed by single-molecule FRET
Nat Commun, 8:15744-15744, 2017

PubMed Abstract: Human myxovirus resistance protein 1 (MxA) restricts a wide range of viruses and is closely related to the membrane-remodelling GTPase dynamin. The functions of MxA rely on domain rearrangements coupled with GTP hydrolysis cycles. To gain insight into this process, we studied real-time domain dynamics of MxA by single-molecule fluorescence resonance energy transfer. We find that the GTPase domain-bundle-signalling-element (BSE) region can adopt either an 'open' or a 'closed' conformation in all nucleotide-loading conditions. Whereas the open conformation is preferred in nucleotide-free, GDP·AlF-bound and GDP-bound forms, loading of GTP activates the relative movement between the two domains and alters the conformational preference to the 'closed' state. Moreover, frequent relative movement was observed between BSE and stalk via hinge 1. On the basis of these results, we suggest how MxA molecules within a helical polymer collectively generate a stable torque through random GTP hydrolysis cycles. Our study provides mechanistic insights into fundamental cellular events such as viral resistance and endocytosis.

PubMed: 28548099
DOI: 10.1038/ncomms15744

Experimental method

X-RAY DIFFRACTION (2.896 Å)