Extending the half-life of a Fab fragment through generation of a humanised anti-Human Serum Albumin (HSA) Fv domain: an investigation into the correlation between affinity and serum half-life

Summary for 5FUO

Related5FUC 5FUZ
DescriptorSERUM ALBUMIN, FAB HEAVY CHAIN, FAB LIGHT CHAIN (3 entities in total)
Functional Keywordsimmune system, anti-albumin, fab fragment, serum half-life, fcrn, human serum albumin
Biological sourceHOMO SAPIENS (HUMAN)
Cellular locationSecreted P02768
Total number of polymer chains3
Total molecular weight114892.04
Adams, R.,Ceska, T. (deposition date: 2016-01-28, release date: 2016-06-29, Last modification date: 2016-11-09)
Primary citation
Adams, R.,Griffin, L.,Compson, J.E.,Jairaj, M.,Baker, T.,Ceska, T.,West, S.,Zaccheo, O.,Dave, E.,Lawson, A.D.G.,Humphreys, D.P.,Heywood, S.
Extending the Half-Life of a Fab Fragment Through Generation of a Humanized Anti-Human Serum Albumin Fv Domain: An Investigation Into the Correlation between Affinity and Serum Half-Life.
Mabs, 8:1336-, 2016
PubMed: 27315033 (PDB entries with the same primary citation)
DOI: 10.1080/19420862.2016.1185581
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.245273.1%6.6%1.1%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 5fuo
no rotation
Molmil generated image of 5fuo
rotated about x axis by 90°
Molmil generated image of 5fuo
rotated about y axis by 90°