5FUO
Extending the half-life of a Fab fragment through generation of a humanised anti-Human Serum Albumin (HSA) Fv domain: an investigation into the correlation between affinity and serum half-life
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-03-23 |
Detector | DECTRIS PILATUS |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 217.640, 217.640, 78.650 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 3.600 |
R-factor | 0.2151 |
Rwork | 0.215 |
R-free | 0.25360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4G03 AND FAB WWPDB SUBMISSION CODE 62161 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.442 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.790 |
High resolution limit [Å] | 3.580 | 3.580 |
Rmerge | 0.010 | 0.040 |
Number of reflections | 46115 | |
<I/σ(I)> | 7.57 | 1.64 |
Completeness [%] | 93.9 | 86.1 |
Redundancy | 2.4 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 1 | 0.1 M CITRIC ACID PH 4.4, 0.1 M DI-SODIUM HYDROGEN PHOSPHATE, 38% V/V ETHANOL, 5% V/V POLYETHYLENE GLYCOL 1000 |