5F8A
Crystal structure of the ternary EcoRV-DNA-Lu complex with uncleaved DNA substrate. Lanthanide binding to EcoRV-DNA complex inhibits cleavage.
Summary for 5F8A
Entry DOI | 10.2210/pdb5f8a/pdb |
Related | 4ORJ 5HLK |
Descriptor | DNA (5'-D(*AP*AP*AP*GP*AP*TP*AP*TP*CP*TP*TP*T)-3'), Type-2 restriction enzyme EcoRV, LUTETIUM (III) ION, ... (6 entities in total) |
Functional Keywords | hydrolase, protein-dna complex, lutetium, hydrolase-dna complex, hydrolase/dna |
Biological source | Escherichia coli More |
Total number of polymer chains | 4 |
Total formula weight | 64959.22 |
Authors | Sangani, S.S.,Kehr, A.D.,Sinha, K.,Rule, G.S.,Jen-Jacobson, L. (deposition date: 2015-12-09, release date: 2016-11-09, Last modification date: 2023-09-27) |
Primary citation | Sinha, K.,Sangani, S.S.,Kehr, A.D.,Rule, G.S.,Jen-Jacobson, L. Metal Ion Binding at the Catalytic Site Induces Widely Distributed Changes in a Sequence Specific Protein-DNA Complex. Biochemistry, 55:6115-6132, 2016 Cited by PubMed: 27786446DOI: 10.1021/acs.biochem.6b00919 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.76 Å) |
Structure validation
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