5F8A

Crystal structure of the ternary EcoRV-DNA-Lu complex with uncleaved DNA substrate. Lanthanide binding to EcoRV-DNA complex inhibits cleavage.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (C, D)	DNA (5'-D(*AP*AP*AP*GP*AP*TP*AP*TP*CP*TP*TP*T)-3')	polymer	12	3660.4	2		synthetic construct
2	C, D (A, B)	Type-2 restriction enzyme EcoRV	polymer	244	28559.2	2	UniProt (P04390) Pfam (PF09233)	Escherichia coli	R.EcoRV,Endonuclease EcoRV,Type II restriction enzyme EcoRV
3	E, H (A, B)	LUTETIUM (III) ION	non-polymer		175.0	2	Chemie (LU)
4	F, I (A, B)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
5	G, J (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
6	K, L, M, N (C, D, A, B)	water	water		18.0	301	Chemie (HOH)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	64439.2
Non-Polymers*	Number of molecules	6
	Total formula weight	520.1
All*	Total formula weight	64959.2

*Water molecules are not included.