5F8A
Crystal structure of the ternary EcoRV-DNA-Lu complex with uncleaved DNA substrate. Lanthanide binding to EcoRV-DNA complex inhibits cleavage.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E DW |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2015-11-18 |
Detector | RIGAKU RAXIS HTC |
Wavelength(s) | 1.54178 |
Spacegroup name | P 1 |
Unit cell lengths | 46.430, 52.860, 65.310 |
Unit cell angles | 70.61, 73.24, 81.81 |
Refinement procedure
Resolution | 36.673 - 1.760 |
R-factor | 0.16402 |
Rwork | 0.160 |
R-free | 0.19914 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1b95 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.574 |
Data reduction software | iMOSFLM (7.2.1) |
Data scaling software | SCALA (CCP4interface 6.5.019) |
Phasing software | PHASER (CCP4interface 6.5.019) |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.673 | 1.850 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.051 | 0.196 |
Number of reflections | 51697 | |
<I/σ(I)> | 14.7 | 4.4 |
Completeness [%] | 93.0 | 88.9 |
Redundancy | 3.9 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 285 | 4.5 mg/mL (monomer), protein(dimer):DNA - 1:1, Enzyme:Well - 3uL:3uL, Well - 500uL of 100 mM HEPES pH 7.4, 8% Ethylene glycol, 4% Polyethylene glycol 8000, 10% glycerol. |