5E67

K103A/K262A double mutant of I-SmaMI

Summary for 5E67

• Entry DOI: 10.2210/pdb5e67/pdb
• Related: 4LOX 5E5O 5E5P 5E5S 5E63
• Descriptor: I-SmaMI LAGLIDADG meganuclease, DNA bottom strand, DNA top strand, ... (7 entities in total)
• Functional Keywords: laglidadg, i-smami, k103a/k262a, hydrolase-dna complex, hydrolase/dna
• Biological source: Sordaria macrospora (strain ATCC MYA-333 / DSM 997 / K(L3346) / K-hell); More
• Total number of polymer chains: 3
• Total formula weight: 49638.22

Authors

Shen, B.W.,Stoddard, B. (deposition date: 2015-10-09, release date: 2016-01-13, Last modification date: 2024-03-06)

• Primary citation: Shen, B.W.,Lambert, A.,Walker, B.C.,Stoddard, B.L.,Kaiser, B.K.; The Structural Basis of Asymmetry in DNA Binding and Cleavage as Exhibited by the I-SmaMI LAGLIDADG Meganuclease.; J.Mol.Biol., 428:206-220, 2016; PubMed: 26705195; DOI: 10.1016/j.jmb.2015.12.005

Experimental method

X-RAY DIFFRACTION (2.2 Å)