5E67
K103A/K262A double mutant of I-SmaMI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-15 |
Detector | RIGAKU SATURN 70 |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.518, 67.326, 98.572 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.200 |
R-factor | 0.18276 |
Rwork | 0.179 |
R-free | 0.25448 |
RMSD bond length | 0.018 |
RMSD bond angle | 2.064 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.619 | |
Number of reflections | 19836 | |
<I/σ(I)> | 17 | 2.3 |
Completeness [%] | 92.0 | 59.2 |
Redundancy | 6.7 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 28 - 32% PEGMME550, HEPES, Mg++ |