5E67
K103A/K262A double mutant of I-SmaMI
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | I-SmaMI LAGLIDADG meganuclease | polymer | 302 | 34036.4 | 1 | UniProt (F7WD42) Pfam (PF00961) In PDB | Sordaria macrospora (strain ATCC MYA-333 / DSM 997 / K(L3346) / K-hell) | |
2 | B | DNA bottom strand | polymer | 25 | 7692.0 | 1 | Synthetic construct | ||
3 | C | DNA top strand | polymer | 25 | 7664.9 | 1 | Synthetic construct | ||
4 | A | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
5 | A | 2-METHOXYETHANOL | non-polymer | 76.1 | 1 | Chemie (MXE) | |||
6 | B | 2-(2-METHOXYETHOXY)ETHANOL | non-polymer | 120.1 | 1 | Chemie (PG0) | |||
7 | water | water | 18.0 | 188 | Chemie (HOH) |
Sequence modifications
A: 1 - 302 (UniProt: F7WD42)
PDB | External Database | Details |
---|---|---|
Ala 103 | Lys 216 | engineered mutation |
Asn 165 | Leu 278 | engineered mutation |
Ala 262 | Lys 375 | engineered mutation |
Gln 267 | Met 380 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 49393.4 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 244.9 | |
All* | Total formula weight | 49638.2 |