5E67
K103A/K262A double mutant of I-SmaMI
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | I-SmaMI LAGLIDADG meganuclease | polymer | 302 | 34036.4 | 1 | UniProt (F7WD42) Pfam (PF00961) | Sordaria macrospora (strain ATCC MYA-333 / DSM 997 / K(L3346) / K-hell) | |
| 2 | B (B) | DNA bottom strand | polymer | 25 | 7692.0 | 1 | Synthetic construct | ||
| 3 | C (C) | DNA top strand | polymer | 25 | 7664.9 | 1 | Synthetic construct | ||
| 4 | D, E (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 5 | F (A) | 2-METHOXYETHANOL | non-polymer | 76.1 | 1 | Chemie (MXE) | |||
| 6 | G (B) | 2-(2-METHOXYETHOXY)ETHANOL | non-polymer | 120.1 | 1 | Chemie (PG0) | |||
| 7 | H, I, J (A, B, C) | water | water | 18.0 | 188 | Chemie (HOH) |
Sequence modifications
A: 1 - 302 (UniProt: F7WD42)
| PDB | External Database | Details |
|---|---|---|
| Ala 103 | Lys 216 | engineered mutation |
| Asn 165 | Leu 278 | engineered mutation |
| Ala 262 | Lys 375 | engineered mutation |
| Gln 267 | Met 380 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 49393.4 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 244.9 | |
| All* | Total formula weight | 49638.2 |
