5E5O

I-SmaMI bound to uncleaved DNA target in the presence of Calcium ions

Summary for 5E5O

• Entry DOI: 10.2210/pdb5e5o/pdb
• Related: 4LOX 5E5P 5E5S 5E63 5E67
• Descriptor: I-SmaMI LAGLIDADG endonuclease, DNA (25-MER), Top strand DNA (25-MER), ... (6 entities in total)
• Functional Keywords: laglidadg, i-smami, complex, uncleaved, hydrolase-dna complex, hydrolase/dna
• Biological source: Sordaria macrospora (strain ATCC MYA-333 / DSM 997 / K(L3346) / K-hell); More
• Total number of polymer chains: 3
• Total formula weight: 50067.35

Authors

Shen, B.W. (deposition date: 2015-10-08, release date: 2016-01-13, Last modification date: 2023-09-27)

• Primary citation: Shen, B.W.,Lambert, A.,Walker, B.C.,Stoddard, B.L.,Kaiser, B.K.; The Structural Basis of Asymmetry in DNA Binding and Cleavage as Exhibited by the I-SmaMI LAGLIDADG Meganuclease.; J.Mol.Biol., 428:206-220, 2016; PubMed: 26705195; DOI: 10.1016/j.jmb.2015.12.005

Experimental method

X-RAY DIFFRACTION (2.36 Å)