5U52

2 helix minimized version of the B-domain from Protein A (Z34C0 bound to IgG1 Fc (monoclinic form)

Summary for 5U52

• Entry DOI: 10.2210/pdb5u52/pdb
• Related: 1L6X 5U4Y 5U66
• Descriptor: IGG1 FC, Mini Z domain, beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• Functional Keywords: 2 helix bundle, protein a, b-domain, igg1 fc, immune system
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 4
• Total formula weight: 58613.48

Authors

Ultsch, M.H.,Eigenbrot, C. (deposition date: 2016-12-06, release date: 2017-05-24, Last modification date: 2024-10-16)

Primary citation

Ultsch, M.,Braisted, A.,Maun, H.R.,Eigenbrot, C.
3-2-1: Structural insights from stepwise shrinkage of a three-helix Fc-binding domain to a single helix.
Protein Eng. Des. Sel., 30:619-625, 2017

PubMed Abstract: The well-studied B-domain from Staphylococcal protein A is a 59 amino acid three-helix bundle that binds the Fc portion of IgG with a dissociation constant of ~35 nM. The B-domain variant bearing a Gly to Ala mutation (=Z-domain) has been the subject of efforts to minimize a domain's size while retaining its function. We report X-ray crystallographic characterization of three steps in such a process using complexes with Fc: the full three-helix Z-domain, a 34 amino acid two-helix version called Z34C and a 13 amino acid single helix stabilized with an exo-helix tether, called LH1.

PubMed: 28475752
DOI: 10.1093/protein/gzx029

Experimental method

X-RAY DIFFRACTION (1.942 Å)