5U52
2 helix minimized version of the B-domain from Protein A (Z34C0 bound to IgG1 Fc (monoclinic form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 170 |
Detector technology | CCD |
Collection date | 1996-10-24 |
Detector | PRINCETON 2K |
Wavelength(s) | 0.95 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.392, 76.390, 66.202 |
Unit cell angles | 90.00, 112.99, 90.00 |
Refinement procedure
Resolution | 19.358 - 1.942 |
R-factor | 0.1919 |
Rwork | 0.190 |
R-free | 0.22750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fc1 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.064 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK (1.5.10) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.020 |
High resolution limit [Å] | 1.942 | 1.942 |
Rmerge | 0.224 | |
Number of reflections | 65918 | |
<I/σ(I)> | 15 | 4.3 |
Completeness [%] | 94.8 | 94.1 |
Redundancy | 1.8 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20% PEG monomethyl ether 550, 0.1M bis-tris propane pH 6.5, 0.1M NaCl, 5mg/mL protein concentration. |