5NFV

Crystal structure of catalytically inactive FnCas12 mutant bound to an R-loop structure containing a pre-crRNA mimic and full-length DNA target

Summary for 5NFV

• Entry DOI: 10.2210/pdb5nfv/pdb
• Descriptor: CRISPR-associated endonuclease Cpf1, pre-crRNA, DNA target strand, ... (7 entities in total)
• Functional Keywords: crispr, cas, cpf1, cas12a, nuclease, genome editing, r-loop, crrna, ruvc, hydrolase
• Biological source: Francisella tularensis subsp. novicida (strain U112); More
• Total number of polymer chains: 4
• Total formula weight: 190495.05

Authors

Swarts, D.C.,van der Oost, J.,Jinek, M. (deposition date: 2017-03-16, release date: 2017-06-14)

• Primary citation: Swarts, D.C.,van der Oost, J.,Jinek, M.; Structural Basis for Guide RNA Processing and Seed-Dependent DNA Targeting by CRISPR-Cas12a.; Mol. Cell, 66:221-233.e4, 2017; PubMed: 28431230; DOI: 10.1016/j.molcel.2017.03.016

Experimental method

X-RAY DIFFRACTION (2.501 Å)