4ZXB

Structure of the human insulin receptor ectodomain, IRDeltabeta construct, in complex with four Fab molecules

Replaces:  2DTGReplaces:  3LOH

Summary for 4ZXB

• Entry DOI: 10.2210/pdb4zxb/pdb
• Descriptor: Fab 83-7 heavy chain, 2-acetamido-2-deoxy-beta-D-glucopyranose, Fab 83-7 light chain, ... (10 entities in total)
• Functional Keywords: receptor tyrosine kinase extracellular domain antibody fragments, hormone receptor-immune system complex, hormone receptor/immune system
• Biological source: Mus musculus; More
• Total number of polymer chains: 5
• Total formula weight: 202011.27

Authors

Croll, T.,Smith, B.J.,Margetts, M.B.,Whittaker, J.,Weiss, M.A.,Ward, C.W.,Lawrence, M.C. (deposition date: 2015-05-20, release date: 2016-02-24, Last modification date: 2024-11-20)

Primary citation

Croll, T.I.,Smith, B.J.,Margetts, M.B.,Whittaker, J.,Weiss, M.A.,Ward, C.W.,Lawrence, M.C.
Higher-Resolution Structure of the Human Insulin Receptor Ectodomain: Multi-Modal Inclusion of the Insert Domain.
Structure, 24:469-476, 2016

PubMed Abstract: Insulin receptor (IR) signaling is critical to controlling nutrient uptake and metabolism. However, only a low-resolution (3.8 Å) structure currently exists for the IR ectodomain, with some segments ill-defined or unmodeled due to disorder. Here, we revise this structure using new diffraction data to 3.3 Å resolution that allow improved modeling of the N-linked glycans, the first and third fibronectin type III domains, and the insert domain. A novel haptic interactive molecular dynamics strategy was used to aid fitting to low-resolution electron density maps. The resulting model provides a foundation for investigation of structural transitions in IR upon ligand binding.

PubMed: 26853939
DOI: 10.1016/j.str.2015.12.014

Experimental method

X-RAY DIFFRACTION (3.3 Å)