4ZXB

Structure of the human insulin receptor ectodomain, IRDeltabeta construct, in complex with four Fab molecules

Replaces:  2DTGReplaces:  3LOH

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Fab 83-7 heavy chain	polymer	219	23414.3	1	UniProt (U5LP42) Pfam (PF07686) Pfam (PF07654) In PDB	Mus musculus
2	B	Fab 83-7 light chain	polymer	219	24385.1	1	UniProt (P01837) Pfam (PF07654) In PDB	Mus musculus
3	C	Fab 83-14 heavy chain	polymer	220	23622.7	1	UniProt (P01865) Pfam (PF07654) In PDB	Mus musculus
4	D	Fab 83-14 light chain	polymer	214	23514.8	1	UniProt (A2P1G9) Pfam (PF07686) Pfam (PF07654) In PDB	Mus musculus
5	E	Insulin receptor	polymer	894	102279.9	1	UniProt (P06213) Pfam (PF00041) Pfam (PF17870) Pfam (PF01030) Pfam (PF00757) In PDB	Homo sapiens
6	F, H, K	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	3	In PDB GlyTouCan (G21290RB)
7	G	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
8	I	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1098.0	1	In PDB GlyTouCan (G39203UC)
9	J, L	alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		367.3	2	In PDB GlyTouCan (G86851RC)
10	E	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)

Sequence modifications

A: 1 - 137 (PDB: 4ZXB)
A: 138 - 219 (UniProt: U5LP42)
B: 1 - 113 (PDB: 4ZXB)
B: 114 - 219 (UniProt: P01837)
C: 1 - 120 (PDB: 4ZXB)
C: 121 - 220 (UniProt: P01865)
D: 1 - 94 (PDB: 4ZXB)
D: 95 - 214 (UniProt: A2P1G9)
E: 1 - 917 (UniProt: P06213)

PDB	External Database	Details
His 144	Tyr 171	engineered mutation
-	Arg 747	deletion
-	Lys 748	deletion
-	Arg 749	deletion
-	Arg 750	deletion
-	Val 758	deletion
-	Thr 759	deletion
-	Val 760	deletion
-	Ala 761	deletion
-	Val 762	deletion
-	Pro 763	deletion
-	Thr 764	deletion
-	Val 765	deletion
-	Ala 766	deletion
-	Ala 767	deletion
-	Phe 768	deletion
-	Pro 769	deletion
-	Asn 770	deletion
-	Thr 771	deletion
-	Ser 772	deletion
-	Ser 773	deletion
-	Thr 774	deletion
-	Ser 775	deletion
-	Val 776	deletion
Ala 750	Pro 777	engineered mutation
Gly 751	Thr 778	engineered mutation
Asn 752	Ser 779	engineered mutation
Asn 753	Pro 780	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	197216.8
Branched	Number of molecules	7
	Total formula weight	4130.9
Non-Polymers*	Number of molecules	3
	Total formula weight	663.6
All*	Total formula weight	202011.3

*Water molecules are not included.