4ZVQ

Caspase-7 Variant 2 (V2) with reprogrammed substrate specificity due to Y230V/W232M/Q276C substitutions bound to VEID inhibitor.

Summary for 4ZVQ

Related4ZVS 4ZVT 4ZVU 4ZVP 4ZVO 4ZVR 1F1J 3EDR
Related PRD IDPRD_000976
DescriptorCaspase-7, Peptide ACE-VAL-GLU-ILE-ASA, ... (4 entities in total)
Functional Keywordsdirected evolution, protease, peptide inhibitor, designed active site specificity, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
Biological sourceHomo sapiens (Human)
More
Cellular locationCytoplasm P55210 P55210
Total number of polymer chains6
Total molecular weight71507.01
Authors
Hill, M.E.,MacPherson, D.J.,Hardy, J.A. (deposition date: 2015-05-18, release date: 2016-04-20, Last modification date: 2017-09-20)
Primary citation
Hill, M.E.,MacPherson, D.J.,Wu, P.,Julien, O.,Wells, J.A.,Hardy, J.A.
Reprogramming Caspase-7 Specificity by Regio-Specific Mutations and Selection Provides Alternate Solutions for Substrate Recognition.
Acs Chem.Biol., 11:1603-1612, 2016
PubMed: 27032039 (PDB entries with the same primary citation)
DOI: 10.1021/acschembio.5b00971
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.5 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.2113001.7%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution