4ZVQ

Caspase-7 Variant 2 (V2) with reprogrammed substrate specificity due to Y230V/W232M/Q276C substitutions bound to VEID inhibitor.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Caspase-7	polymer	198	22189.2	2	UniProt (P55210) Pfam (PF00656) In PDB	Homo sapiens (Human)	CASP-7,Apoptotic protease Mch-3,CMH-1,ICE-like apoptotic protease 3,ICE-LAP3
2	B, D	Caspase-7	polymer	113	13079.8	2	UniProt (P55210) Pfam (PF00656) In PDB	Homo sapiens (Human)	CASP-7,Apoptotic protease Mch-3,CMH-1,ICE-like apoptotic protease 3,ICE-LAP3
3	E, F	Peptide ACE-VAL-GLU-ILE-ASA	polymer	5	484.5	2	BIRD (PRD_000976)	synthetic construct
4		water	water		18.0	99	Chemie (HOH)

Sequence modifications

B, D: 199 - 303 (UniProt: P55210)

PDB	External Database	Details
Val 230	Tyr 263	engineered mutation
Met 232	Trp 265	engineered mutation
Cys 276	Gln 309	engineered mutation
Leu 304	-	expression tag
Glu 305	-	expression tag
His 306	-	expression tag
His 307	-	expression tag
His 308	-	expression tag
His 309	-	expression tag
His 310	-	expression tag
His 311	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	71507.0
All*	Total formula weight	71507.0

*Water molecules are not included.