4XLS

Crystal structure of T. aquaticus transcription initiation complex with CarD containing upstream fork promoter.

Summary for 4XLS

• Entry DOI: 10.2210/pdb4xls/pdb
• Related: 4XAX 4XLN 4XLP 4XLQ 4XLR
• Descriptor: DNA-directed RNA polymerase subunit alpha, MAGNESIUM ION, DNA-directed RNA polymerase subunit beta, ... (10 entities in total)
• Functional Keywords: protein-dna complex, bacterial transcription initiation complex, transcription
• Biological source: Thermus aquaticus; More
• Cellular location: Cytoplasm : Q9EZJ8
• Total number of polymer chains: 18
• Total formula weight: 904642.72

Authors

Bae, B.,Darst, S.A. (deposition date: 2015-01-13, release date: 2015-09-23, Last modification date: 2024-10-30)

Primary citation

Bae, B.,Chen, J.,Davis, E.,Leon, K.,Darst, S.A.,Campbell, E.A.
CarD uses a minor groove wedge mechanism to stabilize the RNA polymerase open promoter complex.
Elife, 4:-, 2015

PubMed Abstract: A key point to regulate gene expression is at transcription initiation, and activators play a major role. CarD, an essential activator in Mycobacterium tuberculosis, is found in many bacteria, including Thermus species, but absent in Escherichia coli. To delineate the molecular mechanism of CarD, we determined crystal structures of Thermus transcription initiation complexes containing CarD. The structures show CarD interacts with the unique DNA topology presented by the upstream double-stranded/single-stranded DNA junction of the transcription bubble. We confirm that our structures correspond to functional activation complexes, and extend our understanding of the role of a conserved CarD Trp residue that serves as a minor groove wedge, preventing collapse of the transcription bubble to stabilize the transcription initiation complex. Unlike E. coli RNAP, many bacterial RNAPs form unstable promoter complexes, explaining the need for CarD.

PubMed: 26349034
DOI: 10.7554/eLife.08505

Experimental method

X-RAY DIFFRACTION (4.01 Å)