4XLS

Crystal structure of T. aquaticus transcription initiation complex with CarD containing upstream fork promoter.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-28
Detector	ADSC QUANTUM 315
Wavelength(s)	1.075
Spacegroup name	P 43 21 2
Unit cell lengths	293.150, 293.150, 539.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.790 - 4.010
R-factor	0.234
Rwork	0.232
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.015
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: DEV_1839))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.790	4.140
High resolution limit [Å]	4.010	4.000
Number of reflections	148127
Completeness [%]		97.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	ammonium sulfate, magnesium chloride, Tris-HCl