4XLQ
Crystal structure of T.aquaticus transcription initiation complex containing upstream fork (-11 base-paired) promoter
Summary for 4XLQ
| Entry DOI | 10.2210/pdb4xlq/pdb |
| Related | 4XAX 4XLN 4XLP 4XLQ 4XLR |
| Descriptor | DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (9 entities in total) |
| Functional Keywords | protein-dna complex, bacterial transcription initiation complex, transcription-dna complex, transcription/dna |
| Biological source | Thermus aquaticus More |
| Total number of polymer chains | 16 |
| Total formula weight | 869245.49 |
| Authors | Bae, B.,Darst, S.A. (deposition date: 2015-01-13, release date: 2015-09-23, Last modification date: 2024-11-20) |
| Primary citation | Bae, B.,Feklistov, A.,Lass-Napiorkowska, A.,Landick, R.,Darst, S.A. Structure of a bacterial RNA polymerase holoenzyme open promoter complex. Elife, 4:-, 2015 Cited by PubMed Abstract: Initiation of transcription is a primary means for controlling gene expression. In bacteria, the RNA polymerase (RNAP) holoenzyme binds and unwinds promoter DNA, forming the transcription bubble of the open promoter complex (RPo). We have determined crystal structures, refined to 4.14 Å-resolution, of RPo containing Thermus aquaticus RNAP holoenzyme and promoter DNA that includes the full transcription bubble. The structures, combined with biochemical analyses, reveal key features supporting the formation and maintenance of the double-strand/single-strand DNA junction at the upstream edge of the -10 element where bubble formation initiates. The results also reveal RNAP interactions with duplex DNA just upstream of the -10 element and potential protein/DNA interactions that direct the DNA template strand into the RNAP active site. Addition of an RNA primer to yield a 4 base-pair post-translocated RNA:DNA hybrid mimics an initially transcribing complex at the point where steric clash initiates abortive initiation and σ(A) dissociation. PubMed: 26349032DOI: 10.7554/eLife.08504 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (4.6 Å) |
Structure validation
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