4XBP

Crystal structure of human 4E10 Fab crystalized in the presence of Phosphatidylethanolamine (06:0 PE)

4XBP の概要

• エントリーDOI: 10.2210/pdb4xbp/pdb
• 関連するPDBエントリー: 4XAW 4XBE 4XBG 4XC1 4XC3 4XCC 4XCE 4XCF 4XCN 4XCY
• 分子名称: 4E10 FAB LIGHT CHAIN, 4E10 Fab heavy chain, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: human 4e10 fab anti hiv-1 gp41, membrane lipids, phosphatidylethanolamine, immune system
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 190396.49

構造登録者

Irimia, A.,Stanfield, R.L.,Wilson, I.A. (登録日: 2014-12-17, 公開日: 2016-02-03, 最終更新日: 2024-11-06)

主引用文献

Irimia, A.,Sarkar, A.,Stanfield, R.L.,Wilson, I.A.
Crystallographic Identification of Lipid as an Integral Component of the Epitope of HIV Broadly Neutralizing Antibody 4E10.
Immunity, 44:21-31, 2016

PubMed Abstract: Numerous studies of the anti-HIV-1 envelope glycoprotein 41 (gp41) broadly neutralizing antibody 4E10 suggest that 4E10 also interacts with membrane lipids, but the antibody regions contacting lipids and its orientation with respect to the viral membrane are unknown. Vaccine immunogens capable of re-eliciting these membrane proximal external region (MPER)-like antibodies may require a lipid component to be successful. We performed a systematic crystallographic study of lipid binding to 4E10 to identify lipids bound by the antibody and the lipid-interacting regions. We identified phosphatidic acid, phosphatidylglycerol, and glycerol phosphate as specific ligands for 4E10 in the crystal structures. 4E10 used its CDRH1 loop to bind the lipid head groups, while its CDRH3 interacted with the hydrophobic lipid tails. Identification of the lipid binding sites on 4E10 may aid design of immunogens for vaccines that include a lipid component in addition to the MPER on gp41 for generation of broadly neutralizing antibodies.

PubMed: 26777395
DOI: 10.1016/j.immuni.2015.12.001

実験手法

X-RAY DIFFRACTION (2.94 Å)