The Crystal Structure of Human Argonaute2 Bound to a Guide and Target RNA Containing Seed Pairing from 2-8 (Long Target)

Summary for 4W5R

Related4W5N 4W5O 4W5Q 4W5T
DescriptorProtein argonaute-2, RNA (5'-R(P*UP*UP*CP*AP*CP*AP*UP*UP*GP*UP*U)-3'), RNA (5'-R(P*AP*AP*UP*GP*UP*GP*AP*AP*A)-3'), ... (7 entities in total)
Functional Keywordsago2, guide, target, seed, rnase, rnai, microrna, hydrolase-rna complex, hydrolase/rna
Biological sourceHomo sapiens (Human)
Cellular locationCytoplasm, P-body  Q9UKV8
Total number of polymer chains3
Total molecular weight108549.78
Schirle, N.T.,MacRae, I.J. (deposition date: 2014-08-18, release date: 2014-11-12, Last modification date: 2017-11-22)
Primary citation
Schirle, N.T.,Sheu-Gruttadauria, J.,MacRae, I.J.
Gene regulation. Structural basis for microRNA targeting.
Science, 346:608-613, 2014
PubMed: 25359968 (PDB entries with the same primary citation)
DOI: 10.1126/science.1258040
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone 0.2334 0.1% 0.4% 5.3% 0.57MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation reportDownload
PDB entries from 2020-10-21