4W5R

The Crystal Structure of Human Argonaute2 Bound to a Guide and Target RNA Containing Seed Pairing from 2-8 (Long Target)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	55.378, 116.184, 69.678
Unit cell angles	90.00, 92.17, 90.00

Refinement procedure

Resolution	69.628 - 2.500
R-factor	0.1966
Rwork	0.195
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4w5o
RMSD bond length	0.006
RMSD bond angle	0.782
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	116.160	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.141
Number of reflections	30428
<I/σ(I)>	9	1.9
Completeness [%]	99.8	99.9
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, Isopropanol, Tris, Phenol, Magnesium