4NGD

Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 12-mer siRNA having 5'-p and UU-3' ends (1.95 Angstrom resolution)

4NGD の概要

• エントリーDOI: 10.2210/pdb4ngd/pdb
• 関連するPDBエントリー: 4NGB 4NGC 4NGF 4NGG 4NH3 4NH5 4NH6 4NHA
• 分子名称: Endoribonuclease Dicer, 5'-R(P*GP*CP*GP*AP*AP*UP*UP*CP*GP*CP*UP*U)-3', SULFATE ION, ... (5 entities in total)
• 機能のキーワード: paz domain, platform domain, connector helix, sirna, rnase iii domain, endoribonuclease, pre-mirna, hydrolase-rna complex, hydrolase/rna
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm : Q9UPY3
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 39121.98

構造登録者

Tian, Y.,Simanshu, D.K.,Patel, D.J. (登録日: 2013-11-01, 公開日: 2014-03-05, 最終更新日: 2023-09-20)

主引用文献

Tian, Y.,Simanshu, D.K.,Ma, J.B.,Park, J.E.,Heo, I.,Kim, V.N.,Patel, D.J.
A Phosphate-Binding Pocket within the Platform-PAZ-Connector Helix Cassette of Human Dicer.
Mol.Cell, 53:606-616, 2014

PubMed Abstract: We have solved two families of crystal structures of the human Dicer "platform-PAZ-connector helix" cassette in complex with small interfering RNAs (siRNAs). The structures possess two adjacently positioned pockets: a 2 nt 3'-overhang-binding pocket within the PAZ domain (3' pocket) and a phosphate-binding pocket within the platform domain (phosphate pocket). One family of complexes contains a knob-like α-helical protrusion, designated "hDicer-specific helix," that separates the two pockets and orients the bound siRNA away from the surface of Dicer, which could be indicative of a product release/transfer state. In the second complex, the helical protrusion is melted/disordered and the bound siRNA is aligned toward the surface of Dicer, suggestive of a cleavage-competent state. These structures allow us to propose that the transition from the cleavage-competent to the postulated product release/transfer state may involve release of the 5'-phosphate from the phosphate pocket while retaining the 3' overhang in the 3' pocket.

PubMed: 24486018
DOI: 10.1016/j.molcel.2014.01.003

実験手法

X-RAY DIFFRACTION (1.958 Å)