4NGD

Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 12-mer siRNA having 5'-p and UU-3' ends (1.95 Angstrom resolution)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	108.710, 84.621, 51.364
Unit cell angles	90.00, 113.33, 90.00

Refinement procedure

Resolution	34.144 - 1.958
R-factor	0.1785
Rwork	0.177
R-free	0.20420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nha
RMSD bond length	0.005
RMSD bond angle	0.960
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.020
High resolution limit [Å]	1.950	4.200	1.950
Rmerge	0.071	0.051	0.284
Number of reflections	28748
<I/σ(I)>	10.2
Completeness [%]	93.9	99.8	70.2
Redundancy	8.8	10.1	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.2 M lithium sulfate, 0.1 M HEPES, pH 7.0-7.5, 16% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K