4NE9

PCSK9 in complex with LDLR peptide

Summary for 4NE9

• Entry DOI: 10.2210/pdb4ne9/pdb
• Related: 2P4E
• Descriptor: Proprotein convertase subtilisin/kexin type 9, Low-density lipoprotein receptor, alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• Functional Keywords: pcsk9, ldl receptor, autocatalytic cleavage, cholesterol metabolism, hydrolase
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 5
• Total formula weight: 152361.05

Authors

Liu, S. (deposition date: 2013-10-28, release date: 2014-09-10, Last modification date: 2020-07-29)

Primary citation

Schroeder, C.I.,Swedberg, J.E.,Withka, J.M.,Rosengren, K.J.,Akcan, M.,Clayton, D.J.,Daly, N.L.,Cheneval, O.,Borzilleri, K.A.,Griffor, M.,Stock, I.,Colless, B.,Walsh, P.,Sunderland, P.,Reyes, A.,Dullea, R.,Ammirati, M.,Liu, S.,McClure, K.F.,Tu, M.,Bhattacharya, S.K.,Liras, S.,Price, D.A.,Craik, D.J.
Design and synthesis of truncated EGF-A peptides that restore LDL-R recycling in the presence of PCSK9 in vitro.
Chem.Biol., 21:284-294, 2014

PubMed Abstract: Disrupting the binding interaction between proprotein convertase (PCSK9) and the epidermal growth factor-like domain A (EGF-A domain) in the low-density lipoprotein receptor (LDL-R) is a promising strategy to promote LDL-R recycling and thereby lower circulating cholesterol levels. In this study, truncated 26 amino acid EGF-A analogs were designed and synthesized, and their structures were analyzed in solution and in complex with PCSK9. The most potent peptide had an increased binding affinity for PCSK9 (KD = 0.6 μM) compared with wild-type EGF-A (KD = 1.2 μM), and the ability to increase LDL-R recycling in the presence of PCSK9 in a cell-based assay.

PubMed: 24440079
DOI: 10.1016/j.chembiol.2013.11.014

Experimental method

X-RAY DIFFRACTION (2.6 Å)